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Bamboo is considered as belonging to the group of geophytes due to the monocarpic pattern of its development. The unique flowering behavior of the most economically important species and the short period of viability seeds mean that the natural means of propagation cannot be depended upon totally. Vegetative propagation in bamboo has been traditionally practiced in different parts of the world. When carried out in propagation units with use of hormones, it can be successfully used for large-scale propagation. A problem in large-scale multiplication is the limitation imposed by availability of plant material. For example,in the method that requires two-node culm cuttings, the number of such cuttings that can be taken from a clump is limited to a few dozens. The large size of the propagules produced by this method is also a limitation in handling and transport over long distances. Tissue culture, an efficient propagation method suitable for large-scale production of planting stock plays an important role in efforts to improve the natural resources of bamboo as in many other plant species. The potential for high rates of multiplication is the greatest advantage of micro propagation ��Nadgir et al., 1984; Prutpongse et al., 1991a, 1992b�� . Production of uniform, disease free plantlets all throughout the year is the other advantage. Tissue culture involves the growth and multiplication of cells, tissues, organs or whole plants on a synthetic nutrient medium under sterile and controlled conditions. The nutrient medium consists not only of a combination of mineral salts, vitamins and sugar which is essential for plant growth but also various types of plant growth regulators ��hormones�� in different combinations and concentrations depending on the type of culture and the plant. How to do bamboo tissue culture is summarized in details as follow. 1 Protocol for Sympodial Bamboo Tissue Culture Sympodial bamboo is non-invasive bamboo which grows best in tropical or subtropical climates. Usually thizome with roots, thizome with culm and roots and thizome with offsets are recommended for sympodial bamboo propagation. However, those traditional methods are not suitable when large-scale bamboo propagation is the objective. Tissue culture is the ideal way to be selected. Bamboo fast propagation is achieved by tissue culture ��Banik, 1991; Zhang et al., 1993; Zhang et al., 2003; Yuan et al., 2009�� . Vegetative parts of young or mature bamboo, such as embryo, culm buds, shoot buds and apical meristem, are induced to produce shoots and roots directly without help of callus. A Young bamboo is that its physiological age is less than ten years ��from the time when the seed germinates to the time when the bamboo is obtained��. Mature bamboo is that its physiological age is at least twenty- five years. 1.1 Selection of Explants Explants selected for bamboo tissue culture should have neotenous features ��juvenile traits��. Since seeds are difficult to obtain for many species, culm buds, shoot buds and apical meristem are often used as explants. Materials from young bamboo are better than those from older plants. Experiment on cutting culture with annual shoots that have just stopped growing during two-year-old and twenty-year-old Dendrocalamus latl[lorus showed that induction rate was 90% and 30% respectively. ����